There is a lab session for Mol Bio of Protein today, and guess what.. Out of the 5 people in my group, only the US gal and myself turned up!! And, there was alot to do..
1) His-Tag Purification of Carbamate Kinase using some nickel column
2) Bradford assay for the crude protein extracts
3) Casting of 2 SDS PAGE gels
And to the lab supervisors surprise, we were pretty much ahead of time and way ahead than the rest of the class and as such, I was also encouraged to perform another set of bradford assay for the fractionated samples, which is supposed to be carried out next weeks.. =)
Interesting.. Not to boast, but I really wondered WHY??!?!!
P.S.: I did the bradford assay for the fractionated samples too.. =)
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